منابع مشابه
Aldehyde dehydrogenase. Covalent intermediate in aldehyde dehydrogenation and ester hydrolysis.
4-trans-(NN-Dimethylamino)cinnamaldehyde (an aldehyde, DACA) and 4-trans-(NN-dimethylamino)cinnamoylimidazole (an amide, DACI) have been shown to be substrates for human aldehyde dehydrogenase (EC 1.2.1.3) which form chromophoric covalent intermediates. The spectra of covalent intermediates from both the cytoplasmic (E1) and mitochondrial (E2) isoenzymes derived from DACA and DACI were compared...
متن کاملHorse Liver Aldehyde Dehydrogenase
Horse liver aldehyde: NAD oxidoreductase (EC 1.2.1.3) has been purified to homogeneity by a procedure consisting of salt fractionation, ion exchange chromatography, and isoelectric focusing. The purif?ed material has a turnover number of 1.85 pmoles of NADH per min per mg of protein when assayed at pH 9.0 with propionaldehyde as substrate. Values obtained for the molecular weight of the native ...
متن کاملHepatic Aldehyde Oxidase
Previous reports on rabbit liver aldehyde oxidase described the purification and some properties of the enzyme (1) and the differential inhibition of electron transfer to various acceptors (2). The latter study furnished evidence for the participation of several electron carriers in the internal electron transport sequence of the enzyme. The present communication summarizes the results of studi...
متن کاملFunctions of aldehyde reductases.
dehydrogenases. The same effects obtained when e N A D + is bound to binary complexes confirm the transfer of the coenzyme to a more hydrophobic environment, but also indicate that the coenzyme is bound to ODH in an open conformation, as found in other dehydrogenases. The high degree of conformational rigidity of bound coenzyme were deduced on the one hand from the negligible temperature coeffi...
متن کاملHuman Liver Aldehyde Dehydrogenase
Human liver aldehyde dehydrogenase has been found to be capable of hydrolyzing p-nitrophenyl esters. Esterase and dehydrogenase activities exhibited identical ion exchange and affinity properties, indicating that the same protein catalyzes both reactions. Competitive inhibition of esterase activity by glyceraldehyde and chloral hydrate furnished evidence that p-nitrophenyl acetate was hydrolyze...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1937
ISSN: 0306-3283
DOI: 10.1042/bj0311347